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SOC Outgrowth Medium
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SOC Outgrowth Medium
SOC Outgrowth Medium from New England Biolabs
(2)
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Citations:
(49)
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Supplier Page from
New England Biolabs for
SOC Outgrowth Medium
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Description
Add SOC to freshly transformed E.coli cells and incubate for up to 1 hour before plating on selective media.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
SOC Outgrowth Medium
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
B9020S
Quantity
100 ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Temperature
4 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (49)
(1)
Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations
Frontiers in Bioengineering and Biotechnology
July 17, 2023
Herbert M. Huttanus, Ellin-Kristina H. Triola, Jeanette C. Velasquez-Guzman, Sang-Min Shin, Rommel S. Granja-Travez, Anmoldeep Singh, Taraka Dale, Ramesh K. Jha
Assembled plasmid DNA library, user supplied.•
SOC Outgrowth Medium
(
New England Biolabs
, cat. no.
B9020S
,• Bacterial growth and selection medium (liquid and agar) e.g., Luria Broth Base (
Thermo Fisher Scientific
,
(2)
Fibertools: fast and accurate DNA-m6A calling using single-molecule long-read sequencing
bioRxiv
July 12, 2023
Anupama Jha, Stephanie C. Bohaczuk, Yizi Mao, Jane Ranchalis, Benjamin J. Mallory, Alan T. Min, Morgan O. Hamm, Elliott Swanson, Connor Finkbeiner, Tony Li,
et al.
back on ice for 5 minutes. Following
incubation
, 950 μL of room temperature SOC media (
NEB
cat. no.
B9020S
) was added, and the cells were allowed to outgrow for 1 hour in the absence of selection. 50 μL of the
(3)
Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids
Cell Reports Medicine
May 16, 2023
Long T. Nguyen, Santosh R. Rananaware, Lilia G. Yang, Nicolas C. Macaluso, Julio E. Ocana-Ortiz, Katelyn S. Meister, Brianna L.M. Pizzano, Luke Samuel W. Sandoval, Raymond C. Hautamaki, Zoe R. Fang,
et al.
Protocols,http://cshprotocols.cshlp.org/content/2015/9/pdb.rec085894.full?rss=1 SOC Medium,
New England Biolabs
,
B9020S
Antifoam 204,Millipore Sigma,Cat# A8311-50ML
NEBuffer 2
⋅1,
New England Biolabs
,Cat#
B9200S
10X Nuclease-Free
(4)
Effect of mTOR inhibitors on sodium taurocholate cotransporting polypeptide (NTCP) function in vitro
Frontiers in Pharmacology
March 22, 2023
Chitra Saran, Henry Ho, Paavo Honkakoski, Kim L. R. Brouwer
ng of the Flp recombinase expression plasmid pOG44 (Invitrogen, CA) in super-optimal broth (S.O.C.; #
B9020S
,
New England Biolabs
) using the manufacturer’s protocol. Ampicillin-resistant bacterial colonies were
(5)
Coordination of bacterial cell wall and outer membrane biosynthesis
Nature
March 1, 2023
Hummels KR, Berry SP, Li Z, Taguchi A, Min JK, Walker S, Marks DS, Bernhardt TG.
µl of
NEB-5-alpha electrocompetent E. coli
(
NEB
C2989K
), and recovered in
SOC outgrowth medium
(
NEB
B9020S
), and transformants were selected by plating the outgrowth onto LB agar supplemented with gentamycin.
(6)
Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assay
Cell Genomics
January 11, 2023
Kousuke Mouri, Hannah B. Dewey, Rodrigo Castro, Daniel Berenzy, Susan Kales, Ryan Tewhey
efficiency. Library E was transformed into 5-alpha
E.coli
(
NEB
,
C2987H
), recovered in SOC medium (
NEB
,
B9020S
) and incubated at 37°C for 1 h then diluted and plated onto LB agar supplemented with 100 μg/mL of carbenicillin
(7)
An Infectious Bursal Disease Virus (IBDV) Reverse Genetics Rescue System and Neutralization Assay in Chicken B Cells
Current Protocols
January 9, 2023
Vishwanatha R. A. P. Reddy, Salik Nazki, Amin Asfor, Andrew J. Broadbent
England Biolabs:
NEB
® 5‐alpha competent
E. coli
;
C2984H
, high efficiency)SOC medium (
New England Biolabs
:
B9020S
)LB agar plate supplemented with 5 μg/ml ampicillinLB broth supplemented with 5 μg/ml ampicillin (Gibco,
(8)
Adaptation delay causes a burst of mutations in bacteria responding to oxidative stress
EMBO reports
January 9, 2023
Valentine Lagage, Victor Chen, Stephan Uphoff
kV electric pulse for 5 ms, Bio‐Rad micropulser). Cells were recovered in
SOC outgrowth medium
(
NEB
B9020S
) for 1 h at 37°C before selection on kanamycin plates. pKD46 plasmid was cured by streaking single colonies
(9)
Genome-wide base editor screen identifies regulators of protein abundance in yeast
Elife
November 3, 2022
Olga T Schubert, Joshua S Bloom, Meru J Sadhu, Leonid Kruglyak
(
Bio-Rad Laboratories
#1652100). After 60 min of growth in liquid recovery media (SOC outgrowth media,
NEB
#
B9020S
), cells were plated onto five 150 mm LB agar plates supplemented with ampicillin/carbenicillin (100
(10)
Functional expression of diverse post-translational peptide-modifying enzymes in Escherichia coli under uniform expression and purification conditions
PLOS ONE
September 19, 2022
Emerson Glassey, Andrew M. King, Daniel A. Anderson, Zhengan Zhang, Christopher A. Voigt
liquid media (S0210, Teknova, Hollister, CA, USA) was used for making competent cells. SOC liquid media (
B9020S
,
New England BioLabs
, Iwsich, MA, USA) was used for outgrowth. Cells were induced with the following
(11)
Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems
STAR Protocols
September 16, 2022
Zhiying Zhao, Jan-Fang Cheng, Yasuo Yoshikuni
High-Fidelity PCR Master
Mix (2×),New England Biolabs,Cat# M0513S SOC medium,
New England Biolabs
,Cat#
B9020S
Sybr Safe,
Invitrogen
,Cat#
S33102
"TAE, 50×",Growcells,Cat# MRGF-4210 Trace mineral solution,ATCC,Cat#
(12)
Polymeric nanoparticles for dual-targeted theranostic gene delivery to hepatocellular carcinoma
Science Advances
July 22, 2022
Hannah J. Vaughan et al.
of the
ligation
product.
Bacteria
were cultured in 450 μl of
SOC outgrowth medium
(
NEB
, catalog no.
B9020S
) for 1 hour at 37°C. The full 500 μl of the
bacteria
suspension was streaked in an LB agar plate with
(13)
Heterologous expression and purification of encapsulins in Streptomyces coelicolor
MethodsX
July 16, 2022
Michael P. Andreas, Tobias W. Giessen
(
Invitrogen
, catalog number
18427013
).(14)
SOC outgrowth medium
(
New England Biolabs
, catalog number:
B9020S
).(15)LB agar, Miller powder (Fisher Scientific, catalog number: BP1425-2).(16)LB broth, Miller mix (Fisher
(14)
Clamping, Bending, and Twisting Inter-Domain Motions in the Misfold-Recognising Portion of UDP-Glucose: Glycoprotein Glucosyl-Transferase
SSRN Electronic Journal
March 30, 2022
Carlos P. Modenutti et al.
transfection reagent
ThermoFisher Scientific Cat# 16447100 SOC Media
New England Biolabs
Cat#
B9020S
Kifunensine
Cayman Chemical
Cat# 109944-15-2
Carbenicillin
Sigma-Aldrich
Cat# C1389 CtUGGT Ref.
(15)
XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coli
STAR Protocols
December 17, 2021
Chirangini Pukhrambam, Irina O. Vvedenskaya, Bryce E. Nickels
Biotech,Cat#R-120-25 H-Bpa-OH,Bachem,Cat#F-2800 IPTG Gold,Biotech,Cat#I2481C50
SOC Outgrowth Medium
,
NEB
,Cat#
B9020S
Lysozyme Egg White,Gold Biotech,Cat#L-040-10 "Glycerol, nuclease free",VWR,Cat#EM 4750 Tris base
(16)
Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications
STAR protocols
December 17, 2021
Kumar A, Lee SJ, Liu Q, Chan AKN, Pokharel SP, Yu J, Chen CW, Swaminathan S.
Memorial Institute)-1640,
Thermo Fisher Scientific
,Cat#
11875119
SOS medium,
New England Biolabs
,Cat#
B9020S
Terrific Broth
(granulated),
Fisher Scientific
,Cat# BP9728-500
PowerUp SYBR Green Master Mix
,
Thermo Fisher Scientific
(17)
Assembly of Longâ€Adapter Singleâ€Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA Targets
Current Protocols
December 2, 2021
Lamia Chkaiban, Lorenzo Tosi, Biju Parekkadan
(
Thermo Fischer Scientific
, cat. no.
15585011
)
SOC outgrowth medium
(
New England BioLabs
, cat. no.
B9020S
) PureLink Quick Plasmid Miniprep Kit (thermos Scientific, cat. no. K210010) Difco,
LB Broth Miller
(18)
Selection for constrained peptides that bind to a single target protein
Nature Communications
December 1, 2021
Andrew M. King et al.
maintenance. SOB liquid media (S0210, Teknova, CA) was used for making competent cells. SOC liquid media (
B9020S
,
New England BioLabs
, MA) was used for outgrowth. Unless noted otherwise, cells were induced with the
(19)
Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming
Cell Metabolism
October 5, 2021
Samantha M. Morrissey et al.
K1876-1G Lennox LB microbial growth medium,
Sigma
,Cat#
L3022
SOC outgrowth medium
,
New England Biolabs
,Cat#
B9020S
Critical Commercial
Assay
,Critical Commercial
Assay
,Critical Commercial
Assay
Exo-Check Exosome Antibody
(20)
Genome-wide functional screen of 3′UTR variants uncovers causal variants for human disease and evolution
Cell
September 30, 2021
Dustin Griesemer et al.
200 ohm, 25 μF). Electroporated
bacteria
were immediately split into five 1 mL aliquots of SOC (
NEB
,
B9020S
) and recovered for 1 hour at 37°C then independently expanded in 1L of LB supplemented with 50 μg/mL
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Reviews
Write a Review
(1)
1/20/2023
Easy Cloning with SOC Outgrowth Medium
Scarlette CASSAL
I use SOC Outgrowth Medium to allow my bacteria to recover and give them time to express the antibiotic gene resistance after transformation by heat shock.
(1)
10/10/2019
Bacterial Cell Recovery Growth Media
Atul Singh
I used this outgrowth recovery media after each transformed cell. Earlier, I used LB medium for recovery, but this NEB SOC provided best results in comparison to LB.
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SOC Outgrowth Medium from New England Biolabs
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